To determine the immunomodulatory properties of Tridax procumbens Linn., and also to check whether significant potentiation in immunomodulation occurs or not with the combination of Lavemisole. Immunomodulatory potential of methanolic extract of Tridax procumbens (METP) (200mg/kg and 400 mg/kg B) and its combination with Lavemisole (50 mg/kg BW) were determined by the effect Cyclophosphamide myelosupression, on leukocyte count and spleen weight, In vivo phagocytosis (carbon clearance method), determination of cellular immune response, haemagglutination antibody titre using sheep red blood cells (SRBC). In addition, the effect of the extract on Systemic Anaphylactic reaction was also measured. Administration of cyclophosphamide reduced the TLC in control animals. Pretreatment of animals with METP and in combination with Lavemisole for 10 days before cyclophosphamide administration produced a significant increase (P<0.01) in TLC and % neutrophil. Leukocyte count and phagocytic index (carbon clearance) was increased significantly with the treatment of METP (400 mg/kg b.w.) compared to control (P<0.05). Lavemisole (50 mg/kg b.w.) in combination with METP (200 mg/kg b.w.) showed highly significant increase of leukocyte count and phagocytic index compared to control (P<0.01). Same groups also showed positive significant action on spleen weight. In cellular immune response study, an enhancement in foot pad thickness was observed when compared to control group. Neither of the two doses of METP showed significant increase in HA titre value and antibody secreting cells of mouse spleen. However, combination of Lavemisole (50 mg/kg b.w.) and METP (200 mg/kg b.w.) showed significant increase (P<0.05) in HA titre and antibody secreting cells of mouse spleen value compared to vehicle control. In systemic anaphylaxis reaction test, results indicate a positive effect on anaphylaxis with the treatment of METP in both doses and in combination with Lavemisole. The present study has shown the immunomodulatory activity of methanolic extract of Tridax procumbens L. at the dose 400 mg/kg BW. T. procumbens L. in combination with zinc has shown potentiation of immunomodulatory activity in all aspects of the study.
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